Toxicological Effects of Colloidal Silver Nanoparticles on Rat Health: Assessing Physiological, Hematological, Biochemical, and Behavioral Parameters

Authors

DOI:

https://doi.org/10.62497/IRABCS.2024.46

Keywords:

AgNPs, assessment, colloidal AgNPs, cognitive function, liver enzyme alterations, multidimensional assessment, nanotechnology, rat health, regulatory decisions, silver nanoparticles, toxicity

Abstract

Introduction: Nanotechnology, a rapidly evolving field, has introduced silver nanoparticles (AgNPs) as promising materials with diverse applications. Their unique properties have revolutionized industries, raising concerns about potential health implications. This study aims to comprehensively evaluate the toxicological effects of AgNPs on rat health through a multidimensional approach, encompassing physiological, hematological, biochemical, and behavioral assessments.

Methodology: Colloidal AgNPs with a minimum purity of 99.98% were synthesized using an inductive coupled plasma (ICP) method. Sixty male specific-pathogen-free (SPF) Wistar rats were exposed to low (10 μg/kg/day) and high (100 μg/kg/day) doses of AgNPs, alongside a control group. Throughout the exposure period, comprehensive assessments, including body weight changes, food intake, water consumption, hematological and biochemical parameters, and behavioral evaluations, were systematically conducted. The statistical analysis employed a combination of one-way ANOVA and Kruskal-Wallis tests for robust data interpretation.

Results: Rats exposed to low (10 μg/kg/day) and high (100 μg/kg/day) doses of AgNPs displayed no significant changes in food intake, water consumption, or blood parameters compared to the control group. However, a notable reduction in body weight was observed in the high-dose group, suggesting a potential dose-dependent impact. Biochemical analyses indicated no significant differences in liver and kidney function parameters for the low-dose group, but the high-dose group exhibited potential elevation in liver enzymes, necessitating further scrutiny. Behavioral assessments revealed no significant alterations in open-field behavior or cognitive function, indicating minimal impact on exploratory and cognitive abilities within the tested doses.

Conclusion: AgNPs demonstrated minimal impact on certain parameters, the high-dose group exhibited notable body weight reduction and potential liver enzyme alterations, warranting further investigation. Behavioral assessments indicated no significant cognitive effects. These findings emphasize the importance of continued research for informed regulatory decisions and the safe utilization of AgNPs in consumer products.

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Author Biographies

  • Roheena Rehman, University of Peshawar, Peshawar

    M.Phil Scholar, Center of Microbiology and Biotechnology

  • Faiza Hayat, Institute of Biotechnology and Genetic Engineering, University of Agriculture, Peshawar

    Ph.D Biotechnology

References

Devi L, Kushwaha P, Ansari TM, Kumar A, Rao A. Recent trends in biologically synthesized metal nanoparticles and their biomedical applications: a review. Biological Trace Element Research. 2023 Oct 25:1-7. https://link.springer.com/article/10.1007/s12011-023-03920-9

Ali M, Ullah M, Khan MM, Rasheed A, Khan M, Aswad S, Zaman MQ. Role of AgNPs in Food Surface Packaging: Effects of AgNPs on Packaging. Biological Sciences-PJSIR. 2023 Mar 17;66(1):65-73. https://v2.pjsir.org/index.php/biological-sciences/article/view/3009

Pinto RJ, Nasirpour M, Carrola J, Oliveira H, Freire CS, Duarte IF. Antimicrobial properties and therapeutic applications of silver nanoparticles and nanocomposites. InAntimicrobial Nanoarchitectonics 2017 Jan 1 (pp. 223-259). Elsevier. https://www.sciencedirect.com/science/article/pii/B9780323527330000094

Oser BL. The rat as a model for human toxicological evaluation. Journal of Toxicology and Environmental Health, Part A Current Issues. 1981 Oct 1;8(4):521-42.

Krishnan PD, Banas D, Durai RD, Kabanov D, Hosnedlova B, Kepinska M, Fernandez C, Ruttkay-Nedecky B, Nguyen HV, Farid A, Sochor J. Silver nanomaterials for wound dressing applications. Pharmaceutics. 2020 Aug 28;12(9):821. https://www.mdpi.com/1999-4923/12/9/821

Rasheed S, Ahmad N, ul Haq MA, Ahmad W, Hussain D. Dual-mode highly selective colorimetric and smartphone-based paper sensors utilizing silver nanoparticles for ultra-trace level omeprazole detection in complex matrices. Journal of Industrial and Engineering Chemistry. 2023 Dec 25;128:450-8. https://doi.org/10.1016/j.jiec.2023.08.009

Nge TT, Nogi M, Suganuma K. Electrical functionality of inkjet-printed silver nanoparticle conductive tracks on nanostructured paper compared with those on plastic substrates. Journal of Materials Chemistry C. 2013;1(34):5235-43. https://pubs.rsc.org/en/content/articlehtml/2013/tc/c3tc31220h

Ferdous Z, Nemmar A. Health impact of silver nanoparticles: a review of the biodistribution and toxicity following various routes of exposure. International journal of molecular sciences. 2020 Mar 30;21(7):2375. https://www.mdpi.com/1422-0067/21/7/2375

Jiravova J, Tomankova KB, Harvanova M, Malina L, Malohlava J, Luhova L, Panacek A, Manisova B, Kolarova H. The effect of silver nanoparticles and silver ions on mammalian and plant cells in vitro. Food and chemical toxicology. 2016 Oct 1;96:50-61. https://www.sciencedirect.com/science/article/pii/S0278691516302356

Xu L, Wang YY, Huang J, Chen CY, Wang ZX, Xie H. Silver nanoparticles: Synthesis, medical applications and biosafety. Theranostics. 2020;10(20):8996. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7415816/

Ellenbroek B, Youn J. Rodent models in neuroscience research: is it a rat race?. Disease models & mechanisms. 2016 Oct 1;9(10):1079-87. https://journals.biologists.com/dmm/article/9/10/1079/3833

Dwinell MR, Lazar J, Geurts AM. The emerging role for rat models in gene discovery. Mammalian Genome. 2011 Aug;22(7):466-75. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643810/

Clements PJ, Bolon B, McInnes E, Mukaratirwa S, Scudamore C. Animal Models in Toxicologic Research: Rodents. InHaschek and Rousseaux's Handbook of Toxicologic Pathology 2022 Jan 1 (pp. 653-694). Academic Press. https://www.sciencedirect.com/science/article/pii/B978012821044400011X

Shahare B, Yashpal M, Gajendra. Toxic effects of repeated oral exposure of silver nanoparticles on small intestine mucosa of mice. Toxicology mechanisms and methods. 2013 Mar 1;23(3):161-7. https://scholar.archive.org/work/tdsuj24xifcitacgmlw47chgnm/access/wayback/http://ugcdskpdf.unipune.ac.in/Journal/uploads/BL/BL100288-A-1.pdf

Park EJ, Bae E, Yi J, Kim Y, Choi K, Lee SH, Yoon J, Lee BC, Park K. Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nanoparticles. Environmental toxicology and pharmacology. 2010 Sep 1;30(2):162-8. https://www.sciencedirect.com/science/article/pii/S1382668910000955

Ryan J, Jacob P, Lee A, Gagnon Z, Pavel IE. Biodistribution and toxicity of antimicrobial ionic silver (Ag+) and silver nanoparticle (AgNP+) species after oral exposure, in Sprague-Dawley rats. Food and Chemical Toxicology. 2022 Aug 1;166:113228. https://www.sciencedirect.com/science/article/am/pii/S0278691522004264

Garcia T, Lafuente D, Blanco J, Sánchez DJ, Sirvent JJ, Domingo JL, Gómez M. Oral subchronic exposure to silver nanoparticles in rats. Food and Chemical Toxicology. 2016 Jun 1;92:177-87. https://www.academia.edu/download/85716040/j.fct.2016.04.01020220509-1-sjtgr1.pdf

Ghareeb OA. Toxic effect of silver nanoparticles on some hematological parameters and possible preventive role of Moringa oleifera: in vivo. Annals of the Romanian Society for Cell Biology. 2021 May 3:13796-801. https://annalsofrscb.ro/index.php/journal/article/download/4505/3618

Al-Baker AA, Al-Kshab AA, Ismail HK, Ashwaq AH. Effect of silver nanoparticles on some blood parameters in rats. Iraqi J Vet Sci. 2020 Jul 1;34(2):389-95. https://www.iasj.net/iasj/download/935c80b541788e01

Tiwari DK, Jin T, Behari J. Dose-dependent in-vivo toxicity assessment of silver nanoparticle in Wistar rats. Toxicology mechanisms and methods. 2011 Jan 1;21(1):13-24. https://doi.org/10.3109/15376516.2010.529184

Lee JH, Gulumian M, Faustman EM, Workman T, Jeon K, Yu IJ. Blood biochemical and hematological study after subacute intravenous injection of gold and silver nanoparticles and coadministered gold and silver nanoparticles of similar sizes. BioMed research international. 2018 Jul 22;2018. https://www.hindawi.com/journals/bmri/2018/8460910/abs/

Zamil RY. The Effects of Different Concentrations of Sliver Nanoparticles on the Kidneys of Male Albino Mice. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS). 2018;50(1):190-203. https://www.academia.edu/download/105749653/1613.pdf

Safaei F, Farimaneh J, Rajabi Mohammad Abad A, Iranmanesh E, Arabpour F, Doostishoar F, Taherizadeh Z. The effect of silver nanoparticles on learning and memory in rodents:" a systematic review". Journal of Occupational Medicine and Toxicology. 2023 Aug 1;18(1):15. https://doi.org/10.1186%2Fs12995-023-00381-7

Węsierska M, Dziendzikowska K, Gromadzka-Ostrowska J, Dudek J, Polkowska-Motrenko H, Audinot JN, Gutleb AC, Lankoff A, Kruszewski M. Silver ions are responsible for memory impairment induced by oral administration of silver nanoparticles. Toxicology letters. 2018 Jun 15;290:133-44. https://www.sciencedirect.com/science/article/pii/S0378427418301061

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Published

07/02/2024

How to Cite

1.
Rehman R, Khanam S, Khan Y, Hayat F. Toxicological Effects of Colloidal Silver Nanoparticles on Rat Health: Assessing Physiological, Hematological, Biochemical, and Behavioral Parameters. IRABCS [Internet]. 2024 Jul. 2 [cited 2024 Dec. 13];2(1):101-7. Available from: https://irabcs.com/ojs/article/view/46

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